Potyviridae and CP) and a small second ORF

Potyviridae infects a wide variety of plants and those infections are
significant constraint to agricultural productivity 1. According to
International Committee on Taxonomy of Viruses (ICTV), Potyviridae is the second largest plant virus family. This family
consists of over 200 viruses divided into 8 genera. All of them are naked
viruses with helical symmetry and having positive sense single-stranded RNA
genome. 5′ end of their genome is linked with viral protein-genome linked (VPg)
and 3′ end of the genome contains poly A tail 2. Macluravirus is a genus of Potyviridae
with the shortest genome (~8 kb) 3, 4. According to ICTV, Macluravirus includes eight virus species
namely Alpinia mosaic virus (AlpMV), Artichoke latent virus (ArLV), Broad-leafed dock virus A (BDVA), Cardamom mosaic virus (CdMV), Chinese
yam necrotic mosaic virus (CYNMV), Maclura
mosaic virus (MacMV), Narcissus
latent virus (NLV) and Yam chlorotic
mosaic virus (YCMV). Apart from these viruses, Large cardamom chirke virus (LCCV) 5 and a new yam infecting
species (Accession number: KM201261) has been identified in India, and their coat
protein (CP) sequences are closely related with other macluraviruses.

The genome of Macluravirus
codes a long open reading frame which is cleaved into nine proteins (HC-Pro,
P3, 7K, CI, 9K, VPg, NIa, NIb and CP) and a small second ORF codes P3N-PIPO 6.
The reported full-length genome sequence of Macluravirus
species possess unique genome composition in Potyviridae, lacks complete P1 and N terminal region of HC-Pro coding
region 3, 4, 7, 8. Another interesting fact about macluraviruses, are
transmitted by aphids though it lacks the helper component region of HC-Pro, essential
for aphid-mediated transmission 9. These uncovered properties of Macluravirus can be addressed by identification
of more new species and its genome analysis. But Macluravirus genus has full-length genome sequence for four viruses
and any genus-specific conserved domain identification need an adequate number
of genome data. Wuhan poty-like virus 1
is a vector-borne poty-like virus genome was identified during the screening of
invertebrate RNA viruses and has been reported as unclassified RNA virus in
NCBI 10. In the present study, genome-wide Potyviridae motif searching, phylogenetic and recombination
analysis have been done to confirm its association with Macluravirus.

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